#C1500) Control standard endotoxin (ACC cat. VMax . 5in (46. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. Published Dec 16, 2020 | Updated Dec 16, 2020. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. On the day of the assay, the plate was equilibrated. Bioz Stars score: 86/100, based on 40 PubMed citations. Integrated stacker option. adjust the reader. x. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…AquaMax® Microplate Washers are configurable with user interchangeable heads for both 96- and 384-well microplate washing applications, including plate or cell washing. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. To test the optical clarity of the various microplates at low wavelengths, one well of each plate was filled with 100 μL (96-well) or 20 μL (384-well) distilled water, and its. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Intuitive graphical user interface makes each assay extremely easy to set. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. M5. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). It offers extremely low absorbance background, excellent. Range of 96, 384-well SBS microplates . NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Optional 12-way manifold) 5032336. Highly selective for dsDNA over ssDNA or RNA. absorbance microplate reader GEA. Supersedes 1. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. Selects either 96-well or 384-well plate for a stand-alone read. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may. #E010) Pyroplate 96-well microplate (ACC cat. to each well. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. #SpectraMax L Config) Methods. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. 5 µL of each sample was pipetted into a 96-well plate for PCR. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader Pred i3x - AV. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 1. 3 OD at 200 nm. Temperature control: 4°C above ambient to 45°C. VMax . Cross-talk <0. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Molecular Devices LLC 96 well plate reader 96 Well Plate Reader, supplied by Molecular Devices LLC, used in various techniques. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data. Lucid Scientific. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Back plate and…. It also integrates with the powerful SoftMax Pro Software for. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Luminescence applications and assays. x. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 003 fmol 0. . 000 OD ± 1. (Molecular Devices cat. $3,450. Uncontrolled Copy for Training and Reference Purposes Only . 1% in white 96- and < 0. measurements in compatible Molecular Devices® microplate readers. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. $488. • 0. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. SoftMax Pro Software minimizes setup time for detection and automates standard curve fitting and sample quantitation. Turn the instrument around so that the back of the instrument is. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. Two high-energy xenon flash lamps: Reading capabilities. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. S. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Fluorescence Microplate Reader Comparison Chart. Ensure that cells reach around 80–90% of confluence on the day of the. These plates are compatible with standard fluorescence microplate readers and are offered in a. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. This can increase throughput and efficiency, thus saving time and space in the lab. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. 0 . Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. Dual injectors for kinetic assays. SoftMax Pro GxP Software version 5. a white 96-well half-area microplate (Corning cat. Mode Plate Reader. Or scan up to 384 wells at any time to determine the best assay conditions. Molecular Devices: FlexStation 3: Multimodal: Limited - monochronometer based: N/A:the iD5 reader’s filter system. Other. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Service parts for these models are not being produced and are no longer in stock. 020 fmol 0. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. Next generation SpectraMax® absorbance microplate readers. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. The. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2. Ultraviolet transparent multiwell plates from GreinerThe Accuris SmartReader UV-Vis (model MR9610) is a welcome addition to any lab that is routinely measuring concentration or absorbance in 96 and/or 384 well plates. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 00. In addition, users can define individual reagents and concentrations to be delivered to each well. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. x. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. 2 OD at 200 nm; this decreased to 0. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Place the microplate in the drawer. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. #ID5-STD) Pierce BCA Protein Assay Kit (ThermoFisher Scientific cat. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Populate the following info under Description. Click Add to create the plate. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Microplate format. 00 USD. #iD5-STD). High tolerance for contaminants. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Next-generation luminescence plate reader. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. Expand Microplates and select the newly created plate. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. S. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Glass microfiber: Use for cell harvesting and binding assays, liquid scintillation counting or high loading, DNA binding and purification; Melt blown polypropylene: Use for pre-filtration; Cellulose acetate: Use for low protein binding,. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The system features 96, 384,. • Easy and gentle immobilization of biotin. #E010) Pyroplate 96-well microplate (ACC cat. Absorbance cuvette port. 1. NanoQuant plate. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 0025 OD from 0. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Features. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. 000 OD ± 1. Published Dec 16, 2020 | Updated Jun 22, 2023. Return to top. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. UV absorbance detection. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. Refurbished. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. When a. From the dropdown Menu, select Wellplates. tools to expand the boundaries of their research. The color touch screen provides a visual user interface, making programming fast and intuitive. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. 340 PC384. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. Plate and Setup Rack Options Screens with selection of up to four Methods shown. d. This reader's dual monochromators for. Our. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. Page 1 of 8 . The Phosentix MicroQC™ Plate is an advanced microplate reader analysis tool that offers exceptional multi-modal functionality. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 1. the Bio-Tek PowerWave™ 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 96-, 384-, and 1536-well microplates. Compare this item. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. x. Compare Tool. U-bottom: 30-40 µl. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. The device was able to achieve 3σ limits of detection of ca. Can read 96 well plates (optional 384 well). The. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. With more than 40 years of plate reader expertise and life science research, we’ve amassed an extensive. Plus 384. Versa Max. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. Performing molecular and cellular analyses in multiwell microplate formats is an important methodology for high-throughput screening, or for boosting throughput generally. maximum-illumination-channels. • Specially formulated optically clear polystyrene and treated to provide protein coating capacity. 1 OD at 230 nm. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. In SoftMax Pro, Dual Read mode was selected. Molecular Devices SpectraMax Plus 384 Absorbance. Selects either 96-well or 384-well plate for a stand-alone read. Absorbance equation. SpectraMax ID5 Plate Reader (Molecular Devices). 190. 9. Conventional technique: subjective, time-consuming. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. Plate chamber design of the SpectraMax category 1 microplate readers. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. Description. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). Two different agonists, acetylcholine and carbachol, were tested. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. 96-, 384-, and 1536-well microplates. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. 00 USD. #3693; n = 4 for each standard). Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The Versamax Microplate Reader is built around a grating monochromator. 96- and 384-well. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. *For Corning UV plates, mean values at 260 nm ranged from 0. No. All trademarks used herein are the property of. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Click on the crosshair and move it so that it is centered over the well image. Growth medium was then replaced with fatty acid uptake solution. Wide range of applications The VersaMax reader covers a wide range of applications: Step 1: Turn on your microplate reader. The 6-well plates were incubated at 37ºC for 9 days before picking. Morphology and fluorescence gates were set to select the best 96 clones secreting IgG antibodies. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Versa Max. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. 45 µm PVDF. 6-384 (96- and 384-well with pipetting) 96-well microplates. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. Pick the best speed, sensitivity, and price for your needs. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. Get Quote . The plate reader can measure multiple readouts, including UV and. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. 046 to 0. 5% at 1. 1 x plates; Instrument dimensions. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. as well as all other Molecular Devices' microplate readers. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Capabilities. 0 Optical system Filters Power requirementAfter reading the ELISA plate on the EMax® Plus Microplate Reader, the R2 value was 0. Microplate optimization and read height optimization were performed for best results. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. We would like to show you a description here but the site won’t allow us. Microplate Capacity. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. 313 reference gain check fail. 5032338. Find our technical support information along with contact details. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Molecular Devices' microplate readers. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Align the two slots on the bottle holder with the two pegs on the rail. The soil was amended with either 1. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Visit the supplier page or submit a quote to learn more. F3/F5 Multi-Mode Microplate ReadersAnalyst® Multimode Reader—one of the following: • Acquest or Analyst AD or HT with at least v. 3% in white 96- and 384. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. robotics-automation-compatible. SpectraDrop High-Throughput Screening (HTS) Kit. . . x. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 40266. Values are averages from 5 clean plates, each filled with 200 µL water/well. (Molecular Devices cat. 0% ± 0. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell a list of plates specifically supported shows, 96, 384, 24, 12, 6 well types. The four standard filters – 405, 450, 492, 620 nm – allow for a wide variety of ELISAs such as PNPP, ABTS, OPD and TMB. 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. 2. 00. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Microplate Readers include the M3, M4, M5, and M5e models. 1 – 2. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). This page contains the. We would like to show you a description here but the site won’t allow us. What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. 100 μL of working reagent was added to each well after the 10-minute preincubation step. Important things to consider when purchasing a multimode microplate reader. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. The VICTOR Nivo. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 300 µL of Detergent Compatible Bradford reagent was added to each well. SpectraMax 250 User. 2 with 1000 cells. )Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. What are the dimensions of the plate? Code. The opaque walls prevent stray light from being detected from neighbouring. Microplate optimization and read height optimization were performed for best results. These include real-time PCR, measuring yields of RNA preparations for microarray experiments, reverse transcription PCR (RT-PCR), differential display PCR,. 5. Working in. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. 0200-6263. FlexIII. Related Products: 96 Well Plate Reader. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. 96 well plate injection time with SpectraMax® L plate reader. Materials.